BRUKER Luxendo社 light-sheet microscopy Products
BRUKER Luxendo社は、2015年にEMBLからスピンオフし設立されました。2017年5月より最先端の科学分析機器を提供するBRUKERグループの傘下となり、益々開発を加速させています。独自のライトシート蛍光顕微鏡装置を開発および製造しています。アプリケーションに重点を置き、使いやすく、世界中の科学者に使用されています。顕微鏡は、生物の薄いスライスのスタックを連続的に照らすことにより、サンプルの光毒性を回避し、光損傷なしで長期間生物を観察することができます。異なるタイプのlight-sheet顕微鏡システムがあり、様々な用途のニーズを満す事ができます。
関連新着情報
- 2024/4/1
- バイオイメージングニュース Vol.3「特集: スフェロイド等のタイムラプスイメージングが可能なライトシート顕微鏡 TruLive3D Imager」を掲載しました。
スフェロイドのような培養細胞などをライトシート顕微鏡を用いてイメージングする際の利点をご紹介いたします。 - 2024/4/1
- 製品ページにPublicationsを追加しました。
- 2024/4/1
- バイオイメージングニュース Vol.1「特集: ライトシート顕微鏡の照明技術(ガルバノスキャナ)」を掲載しました。
ライトシート顕微鏡でガルバノスキャナを用いる2つの利点についてご説明しています。
透明化サンプル用ライトシート顕微鏡 LCS-SPIM
LCS SPIMは透明化サンプルに特化した専用ライトシート顕微鏡です。2本の照明用対物レンズと1本の検出用対物レンズで撮影します。サンプルチャンバー（容器）の大きさは、3種類から選択可能で、最大は30mmx30mmx100mmのクォーツ容器になります。従来のMuVi-SPIM CSでは不可能であったホールボディーや大きな全脳の撮影がカットすることなく可能になりました。高速撮影のオプションも豊富に取り揃え、12mmサイズの全脳を5分で撮影可能です。ホールボディーや、透明化脳、透明化臓器といった大きなサンプルをカットすることなく、高速でスクリーニング的に観察するアプリケーションに最適です。
マルチビューライトシート顕微鏡 MuVi-SPIM LS MuVi-SPIM CS
MuVi-SPIM LSは2本の照明用対物レンズと2本の検出用対物レンズでサンプルを360°照明し、撮影できるライトシート顕微鏡です。これにより、サンプルの回転を必要とせずに、4つの角度から全体を撮影します。また、4つの視野を75fps@2Kx2Kで表示するなど、従来のマルチアングル型ライトシート顕微鏡に比べて大幅に撮影速度が速く、回転させない安定した状態のサンプルを長時間4Dライブイメージングできます。サンプルを置くピエゾステージは高精度な位置調整を行い、ビームスキャン方式で薄く形成されたシート照明は高い分解能を発揮します。
MuVi-SPIM CSは透明化サンプルに特化した専用ライトシート顕微鏡です。2本の照明用対物レンズと2本若しくは1本の高倍率検出用対物レンズで高解像度にて撮影します。サンプルマウントは容易で、3Dプリンターにてカスタマイズ作成されたアームにサンプルを簡易固定し、ロボットアームにてサンプルチャンバーに入れます。大きなサンプルは180度ローテーションして各パートを撮影し、最終的に360度のホールイメージを作成します。
マルチサンプルライブイメージングライトシート顕微鏡 TruLive3D Imager
倒立型のTrueLive3D Imagerは、高速3Dマルチサンプルライブイメージングに最適化されたライトシート顕微鏡です。左右2方向からの励起光照射により、高速、高解像イメージング(255nm)を可能にし、シャドウを最小限に抑えます。最大100サンプルまでのライブイメージングに対応し、マウス初期胚、卵母細胞、スフェロイド、オルガノイド等のタイムラプス、ハイスループットイメージングが可能です。フル環境コントロール装置により、CO2、O2、温度、湿度を管理し、シート光による光ダメージの低減もあり、長時間のライブ観察が可能になりました。
光格子(ラティス) ライトシート顕微鏡 InVi SPIM Lattice Pro
InVi SPIM Lattice Proは、独自のAdvanced Illumination Module (AIM)を使用して様々なビーム形状を提供します。このライトシート顕微鏡はシステムの簡便さと安定性を維持しながら、サンプルの特殊な要件に合わせてビーム形状をカスタマイズ可能でかつインタラクティブに対応できる機能を有しています。高速3Dライブイメージングに必須である極低ダメージ、広い視野、高速性、そして高い空間解像度を実現します。
| Year | JOURNAL | Title | Author(s) | Imaging Platform | Subject | Methodology |
|---|---|---|---|---|---|---|
| 2023 | The EMBO Journal | Embryo-uterine interaction coordinates mouse embryogenesis during implantation | V Bondarenko, M Nikolaev, D Kromm,(..)Takashi Hiiragi | InVi SPIM | Pre-implantaion development, Morphogenesis, Patterning | Mouse, Ex-vivo, Live imaging, Laser ablation |
| 2023 | Journal of Neuroscience | A Cellular Ground Truth to Develop MRI Signatures in Glioma Models by Correlative Light Sheet Microscopy and Atlas-Based Coregistration | K Schregel, L Heinz, J Hunger, C Pan, (..) Michael O. Breckwoldt | |||
| 2023 | Clinical and Translational Medicine | Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer | S Sivakumar, S Lieber, D Librizzi, (..) Sabine Müller-Brüsselbach, Rolf Müller | LCS SPIM | ovarian cancer, metastasis | Basal cell adhesion molecule (BCAM) is a laminin α5 (LAMA5), Biochemical, omics-based and real-time cell assays |
| 2023 | Advanced Science | The Role of PRRC2B in Cerebral Vascular Remodeling Under Acute Hypoxia in Mice | S Li, W Hu, S Gong, P Zhang, J Cheng,(..) Zengqiang Yuan | MuVi SPIM | hypoxia | mouse, high altitude exposure, cerebral vasculature, RNA immunoprcipitation-seq, and transcriptomic co-analysis |
| 2022 | Nature | Periodic formation of epithelial somites from human pluripotent stem cells | M Sanaki-Matsumiya, M Matsuda, N Gritti… Miki Ebisuya | MuVi SPIM | embryonic development, somitogenesis, segmentation clock | Human iPSC cultures, human somitoids, human somitoids, HCR, IHC, timelapses, human somitoids, morphometry |
| 2022 | National Acad Sciences | Dynamics of Drosophila endoderm specification | SE Keenan, M Avdeeva, L Yang… Stanislav Y. Shvartsman | MuVi SPIM | Drosophila 4D imaging, developmental patterning, gene regulatory network | drosophila, Time-lapse microscopy, single-cell, modelling, image analysis |
| 2022 | Mammary Stem Cells: Methods | Modification of Single Cells Within Mouse Mammary Gland Derived Acini via Viral Transduction | LG Del Valle, MG Montero, M Jechlinger | InVi light-sheet | organoid culture optimization | mouse, organoid cultures, primary donor tissue, 3D acini |
| 2022 | Nature | Embryo-scale epithelial buckling forms a propagating furrow that initiates gastrulation | J Fierling, A John, B Delorme, A Torzynski… Matteo Rauzi | biomechanics, tissue buckling, mesoderm internalization | Drosophila , actomyosin contraction, computational models, in toto embryo image analysis and manipulation | |
| 2022 | Cell | An ex vivo system to study cellular dynamics underlying mouse peri-implantation development | Takafumi Ichikawa, Hui Ting Zhang, Laura Panavaite, ... Takashi Hiiragi | peri-implantation embryo development, mechano-chemical interactions | mouse, 3D ex vivo culture | |
| 2022 | https://link.springer.com/protocol/10.1007/978-1-0716-2659-7_7 | |||||
| 2022 | The Lancet | Müller glia fused with adult stem cells undergo neural differentiation in human retinal models | SÀ Bonilla-Pons, S Nakagawa, EG Bahima… Maria Pia Cosma | MuVi SPIM | retina organoids, Müller glia, regeneration | organotypic cultures of human retina, preparations of dissociated cells, microinjection system, qRT-PCR, immunofluorescence, Flow cytometry |
| 2022 | Genome Biology | Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts | T De Coster, H Masset, O Tšuiko, M Catteeuw, Y Zhao… Joris Robert Vermeesch | InVi SPIM | genome segregation, zygotic division | Bovine embryo in vitro production, imaging, genome analysis, Nuclear staining, human biopsies |
| 2022 | iScience | Discovery of a novel SHIP1 agonist that promotes degradation of lipid-laden phagocytic cargo by microglia | C Pedicone, S Fernandes, A Matera, ST Meyer, S Loh…William G. Kerr | InVi SPIM | degradation of Synaptosomes, phagocytic degradation, microglia | artificial intelligence, mice, computational method, CRISPR/Cas9, immunofluorescence, flow cytometry |
| 2022 | STEM CELLS AND REGENERATION | Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids | D Medina-Cano, EK Corrigan, RA Glenn… Thomas Vierbuchen | TrueLive3D Imager | developmental biology, organoids | mouse, pluripotent stem cells, Wnt pathway inhibitors, definitive endoderm and neural organoids,differentiation models |
| 2022 | Science | Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals | A Torres-Méndez, S Pop, S Bonnal, I Almudi, .. Manuel Irimia | MuVi SPIM | phylogeny, evolution, neuronal transcriptomic complexity | neuronal imaging experiments and cell type–specific rescues |
| 2022 | EMBO Reports | PLETHORA‐WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance | RC Burkart, VI Strotmann, GK Kirschner, A Akinci…Yvonne Stahl | MuVi SPIM | stem cell niche | Arabidopsis, lateral root primordium, cloning, staining, Nicotiana benthamiana infiltration, SCN staining, RNA staining, imaging, image analysis |
| 2022 | Developmental Cell | Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster | I Miguel-Escalada, MA Maestro, D Balboa, A Elek… Jorge Ferrer | MuVi SPIM | polygenic disease, pancreas agenesis, enhancer cluster | immunohistochemistry, RT-PCR, Flow cytometry, Seq analysis, ChIP-Seq analysis |
| 2022 | Cell Reports | The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions | CF Hevia, C Engel-Pizcueta, F Udina, C Pujades | MuVi SPIM | hindbrain morphogenesis, Notch signalling, stem cells, neurogenesis | zebrafish, CRISPR-Cas9, ISH, pharmacological treatments, immunohistochemistry, imaging, image analysis |
| 2021 | eLife | Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development | L Zilova, V Weinhardt, T Tavhelidse, C Schlagheck… Joachim Wittbrodt | MuVi SPIM | morphogenesis and differentiation | medaka and zebrafish, embryonic pluripotent stem cells, fluorescent labeling, quantification |
| 2021 | Plant & Cell Physiology | Integration of Cell Growth and Asymmetric Division during Lateral Root Initiation in Arabidopsis thaliana | LM Schütz, M Louveaux, A Vilches Barro…Alexis Maizel | MuVi SPIM | Lateral root formation, periclinal division | Arabidopsis thaliana, four-dimensional reconstruction, lineage tracking, segmentation |
| 2021 | Biophysical Journal | Pinching and pushing: fold formation in the Drosophila dorsal epidermis | V Velagala, JJ Zartman | MuVi SPIM | planar epithelial sheets, morphogenesis, Hedgehog signalling | Drosophila, immunostaining, image processing, image analysis |
| 2021 | Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces | |||||
| 2021 | BMC Dev Biol | The migratory pathways of the cells that form the endocardium, dorsal aortae, and head vasculature in the mouse embryo | C Collart, A Ciccarelli…J. C. Smith | MuVi SPIM | Vasculogenesis, embryogenesis | mouse embryo, single-cell transcriptomics, imaging, OPT |
| 2021 | Developmental Cell | A two-tier junctional mechanism drives simultaneous tissue folding and extension | A John, M Rauzi | MuVi SPIM | adherens junctions, tissue folding and extension, embryogenesis | Drosophila, In toto 4-D imaging, digital reconstruction, data processing, Drug injections, Dendra photoconversion, Cry2 optorecruitment, Actomyosin laser ablation |
| 2021 | ELife | Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach | A Seleit, A Aulehla, A Paix | TruLive 3D Imager | technology Crispr/Cas9, protocol | Crispr/Cas9, medaka, whole-genome sequencing |
| 2021 | Nature | Smad4 controls signaling robustness and morphogenesis by differentially contributing to the Nodal and BMP pathways | L Guglielmi, C Heliot, S Kumar, Y Alexandrov… Caroline S. Hill | MuVi SPIM | morphogens, morphometry | TGF-β family, SMAD4, zebrafish, Nodal, zebrafish, image analysis |
| 2021 | Cell Reports | Endothelial Wnts control mammary epithelial patterning via fibroblast signaling | J Wang, W Song, R Yang, C Li, T Wu, XB Dong, B Zhou… Yi Arial Zeng | MuVi SPIM | endothelial cells, Wnt, fibroblasts | fluorescent mouse, organoid, FACS, immnohistochemistry, RNA-seq |
| 2020 | Semi-automatic generation of tight binary masks and non-convex isosurfaces for quantitative analysis of 3D biological samples | |||||
| 2020 | Front. Plant Sci. | Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis | M Tichá, H Richter, M Ovečka, N Maghelli…Olga Šamajová | MuVi SPIM | Annexin 1, cell biology, lateral root growth | Arabidopsis, transgenic lines, immunoblotting, image analysis, Quantitative PCR |
| 2020 | Elife | Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation | A Alladin, L Chaible, L Garcia del Valle, R Sabine… Martin Jechlinger | InVi SPIM | cancer environment, oncogenes, breast cancer | mouse, 3D acinus cultures, qPCR analysis, image analysis, |
| 2020 | Cell Discovery | Light-sheet fluorescence imaging charts the gastrula origin of vascular endothelial cells in early zebrafish embryos | M Pang, L Bai, W Zong, X Wang, Y Bu, C Xiong… Jing-Wei Xiong | MuVi SPIM | cell lineage map, vascular endothelial cells | zebrafish, alignment, fusion, and extraction all-in-one software (AFEIO) for processing big data, quantitative analysis, photoconversion |
| 2020 | Macromolecular Biosciences | Poly(N‐isopropylacrylamide)‐Based Polymers as Additive for Rapid Generation of Spheroid via Hanging Drop Method | V RA, S Kumari, P Poddar, D Dhara… Souvik Maiti | MuVi SPIM | Multicellular tumor spheroid, protocol, tissue clearing | |
| 2020 | Journal of Biophotonics | Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution | P Matryba, A Wolny, M Pawłowska, ..Jakub Golab | tissue clearing | mouse penis | |
| 2020 | Elife | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy | C Prahst, P Ashrafzadeh, T Mead, A Figueiredo…Katie Bentley | MuVi SPIM | retina, opthalmology, vasculature | mouse, image processing |
| 2020 | Current Biology | Cell death in cells overlying lateral root primordia facilitates organ growth in Arabidopsis | S Escamez, D André, B Sztojka, B Bollhöner, H Hall… Hannele Tuominen | - | lateral root growth, cell death, cell biology | Arabidopsis, Gravitational induction of LRP initiation, Transmission electron microscopy, Light microscopy, Laser-assisted targeted cell elimination, RNA isolation and qPCR |
| 2020 | Front. Bioeng. Biotechnol. | Opsef: Open source python framework for collaborative instance segmentation of bioimages | TM Rasse, R Hollandi, P Horvath | MuVi SPIM | Python framework for deep learning-based instance segmentation | Arabidopsis, image analysis |
| 2020 | Developmental Dynamics | Fusion of airways during avian lung development constitutes a novel mechanism for the formation of continuous lumena in multicellular epithelia | MA Palmer, CM Nelson | MuVi SPIM | avian lung development, epithelium | chicken, lung, photomanipulation |
| 2020 | ALTEX | Incorporation of stem cell-derived astrocytes into neuronal organoids to allow neuro-glial interactions in toxicological studies | M Brüll, AS Spreng, S Gutbier, D Loser, A Krebs… | MuVi SPIM | neuro-glial interactions , astrocytes, organoids | cell culture, proliferation, imaging |
| 2020 | Elife | Accurate and versatile 3D segmentation of plant tissues at cellular resolution | A Wolny, L Cerrone, A Vijayan, R Tofanelli, AV Barro… | MuVi SPIM | PlantSeg, cell segmentation | image analysis |
| 2020 | Current Biology | Axis specification in zebrafish is robust to cell mixing and reveals a regulation of pattern formation by morphogenesis | T Fulton, V Trivedi, A Attardi, K Anlas, C Dingare… | MuVi SPIM | morphogenesis, axis formation, development | zebrafish, explants,Mosaic Labeling, pharmacological inh, HCR, immunohistochemistry |
| 2020 | Developmental Cell | Tissue-scale mechanical coupling reduces morphogenetic noise to ensure precision during epithelial folding | AS Eritano, CL Bromley, AB Albero, L Schütz, FL Wen… | MuVi SPIM | development, epithelium, transcription | Drosophila, laser ablation, optogenetics, modelling |